New method for cancer tretIn patients with peritoneal carcinomatosis cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC) represents a promising treatment strategy.
Here, we studied the role of hyperthermic chemotherapy on heat shock protein (HSP) expression and induction of tumor cell death and surviva HSP70, and HSP90 combined with effects on tumor cell proliferation and chemosensitivity were analyzed in human colon cancer.
Hyperthermic chemotherapy resulted in significant HSP27/HSP70 and HSP90 gene/protein overexpression in analyzed HT-29/SW480/SW620 colon cancer cells and peritoneal metastases from patients displaying amplified expression of proliferation markers, proliferating cell nuclear antigen and antiapoptotic protein Bcl-xL. Moreover, functionally increased chemoresistance against 5-fluorouracil/mitomycin C and oxaliplatin after hyperthermic chemotherapy points to induced survival mechanisms in cancer cells. In conclusion,
the results indicate that intracellular HSP-associated antiapoptotic and proliferative effects after hyperthermic chemotherapy negatively influence beneficial effects of hyperthermic chemotherapy-induced cell death From a total of 63 patients who underwent CRS with additional HIPEC therapy as a closed procedure in our department (October 2008 to April 2011) and available representative tumor tissues before and after HIPEC therapy, 24 patients with isolated PC from colorectal cancer were investigated. Informed consent was obtained preoperatively, as was approved by the local medical ethics committee. Patient data are summarized in Table 1. The HIPEC therapy was performed under specific conditions (60 minutes permanent chemotherapeutical flux via external pump into the abdominal cavity after resection of relevant tumor masses with elevated temperature up to 41°C). Tumor biopsies from peritoneal tumors were taken before and after the HIPEC procedure. Tumor biopsies after a HIPEC procedure were taken from specifically marked peritoneal areas with small peritoneal metastases completely resected after the HIPEC.ment
Here, we studied the role of hyperthermic chemotherapy on heat shock protein (HSP) expression and induction of tumor cell death and surviva HSP70, and HSP90 combined with effects on tumor cell proliferation and chemosensitivity were analyzed in human colon cancer.
Hyperthermic chemotherapy resulted in significant HSP27/HSP70 and HSP90 gene/protein overexpression in analyzed HT-29/SW480/SW620 colon cancer cells and peritoneal metastases from patients displaying amplified expression of proliferation markers, proliferating cell nuclear antigen and antiapoptotic protein Bcl-xL. Moreover, functionally increased chemoresistance against 5-fluorouracil/mitomycin C and oxaliplatin after hyperthermic chemotherapy points to induced survival mechanisms in cancer cells. In conclusion,
the results indicate that intracellular HSP-associated antiapoptotic and proliferative effects after hyperthermic chemotherapy negatively influence beneficial effects of hyperthermic chemotherapy-induced cell death From a total of 63 patients who underwent CRS with additional HIPEC therapy as a closed procedure in our department (October 2008 to April 2011) and available representative tumor tissues before and after HIPEC therapy, 24 patients with isolated PC from colorectal cancer were investigated. Informed consent was obtained preoperatively, as was approved by the local medical ethics committee. Patient data are summarized in Table 1. The HIPEC therapy was performed under specific conditions (60 minutes permanent chemotherapeutical flux via external pump into the abdominal cavity after resection of relevant tumor masses with elevated temperature up to 41°C). Tumor biopsies from peritoneal tumors were taken before and after the HIPEC procedure. Tumor biopsies after a HIPEC procedure were taken from specifically marked peritoneal areas with small peritoneal metastases completely resected after the HIPEC.ment
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